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Cell Culture in Cosmetic Science
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Course data
Course orientation
Announcements
Topic 1
Course orientation
Collecting quick ideas and responses are used to get initial interest from the class.
Lesson Plan
Inspiration: Save Ralph
Topic 1: Introduction to animal tissue culture-Lecture Note
Topic 1: Introduction to animal tissue culture-Handouts
Self-study: Introduction to cell culture
Pair your lab group
Exp. 1: Sterilization and reagent preparation for cell culture
Exp. 2: Aseptic handling and preparation of stock medium from powder
Pre-lab 1 : Sterilization and reagent preparation for cell culture
Pre-lab 2: Aseptic handling and preparation of stock medium from powder
Lab report: Exp. 1
Lab report: Exp. 2
Rubric for Lab Reports
Pre-Lab Grading Rubric
Topic 2
Topic 2: Biology of cultured cells-Lecture note
Topic 2: Biology of cultured cells-Handout
Additional Video for Epithelial Cell Migration 1
Additional Video for Epithelial Cell Migration 2
Self-study: Cell cycle
Self-study: Cell signaling
Self-study: Cell differentiation
Self-study: Telomere and cell senescence
Exp. 3: Preparation of complete culture medium and thawing cell
Pre-lab 3: Preparation of complete culture medium and thawing cell
Lab Report: Exp.3
Topic 3
Topic 3: Aseptic technique-Lecture note
Topic 3: Aseptic technique-Handout
Self-study: Preventing contamination in cell culture laboratory
Exp. 4: Procedure for maintenance and subculture
Pre-lab 4: Procedure for maintenance and subculture
Lab report: Exp.4
Topic 4
Topic 4: Laboratory equipment and material-Lecture note
Topic 4: Laboratory equipment and material-Handout
Exp 5: Procedure for cell counting and viability
Pre-lab 5: Procedure for cell counting and viability
Lab report: Exp.5
Topic 5
Topic 5: Preparation and sterilization-Lecture note
Topic 5: Preparation and sterilization-Handout
Exit Ticket: Preparation and sterilization
Types of Autoclaves (Gravity vs. Vacuum Autoclaves) and Their Advantages
How to use a Steripak™-GP filter to filter/sterilize large volumes of tissue culture media
Knowledge News: How to use 3M CIs and Tape to make sure your sterilizer is working properly
Clonogenic Cell Survival Assay
Exp. 6: Cryopreservation
Pre lab 6: Cryopreservation
Lab report: Exp.6
Topic 6
Topic 6: Media and supplements-Lecture note
Topic 6: Media and supplements-Handout
Exp. 7: Contamination testing in cell culture
Pre lab 7: Contamination testing in cell culture
Lab report: Exp. 7
Topic 7
Topic 7: Contamination-Lecture note
Topic 7: Contamination-Handout
Exp. 8-9: Cytotoxicity assay: MTT assay 1-2 (2 weeks)
Pre lab 8-9: Cytotoxicity assay: MTT assay
Lab report: Exp. 8-9
Topic 8
Topic 8: Safety and bioethics-Lecture note
Topic 8: Safety and bioethics-Handout
Think-Pair-Share: The immortal cells of Henrietta Lacks - Robin Bulleri
Topic 9
Entry ticket: Primary culture
Topic 9: Primary culture-Lecture note
Topic 9: Primary culture- Handout
Exp. 10: Cytotoxicity assay: Clonogenic assay
Pre lab 10: Cytotoxicity assay: Clonogenic assay
Lab report: Exp. 10
Topic 10
Topic 10: Subculture and cell lines-Lecture note
Topic 10: Subculture and cell lines-Handout
Self-study: Subculture and cell lines
Topic 11
Topic 11: Cryopreservation-Lecture note
Topic 11: Cryopreservation-Handout
Topic 12
Topic 12: Quantitation in the culture of animal cells-Lecture note
Topic 12: Quantitation in the culture of animal cells-Handout
Exit ticket: Quantitation in the culture of animal cells
Exp. 11: Antioxidant activity assay in cell culture
Pre lab 11: Antioxidant activity assay in cell culture
Lab report: Exp.11
Lab examination
Final examination-Project planning rubric
Final examination-Self-assessment rubric
Final examination-Peer assessment rubric
Final examination-Final report assessment rubric
Topic 13
Topic 13: Cytotoxicity assay-Lecture note
Topic 13: Cytotoxicity assay-Handout
Collecting quick ideas and responses for the final class.
Exp. 12: Anti-melanogenic activity assay in cell culture
Exp. 13: Anti-tyrosinase assay in cell culture
Pre lab 12:Anti-melanogenic and activity assay in cell culture
Pre lab 13: Anti-tyrosinase assay in cell culture
Lab report: Exp. 12
Lab report: Exp. 13
Final examination (LAB)-Project planning
Topic 14
Exp. 14: Wound healing assay in cell culture
Pre lab 14: Wound healing assay in cell culture
Lab report: Exp. 14
Topic 15
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Semester 2-2567
School of Liberal Arts 2-2567
School of Science 2-2567
School of Management 2-2567
School of Applied Digital Technology 2-2567
School of Agro-Industry 2-2567
School of Law 2-2567
School of Cosmetic Science 2-2567
School of Health Science 2-2567
School of Nursing 2-2567
School of Anti-Aging and Regenerative Medicine 2-2567
School of Medicine 2-2567
School of Dentistry 2-2567
School of Social Innovation 2-2567
School of Sinology 2-2567
School of Integrative Medicine 2-2567
Workshop
MLII-Courses
Home
LMS Support (08.30-17.00)
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- Contact Admin Page
- LMS Line Official
- Email : lms.mlii@mfu.ac.th
- Phone No. 053-917-897 Ext. 8082
LMS Troubleshoot
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Expand
- Google Chrome Ploblem
LMS Manual
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- Manual For Teacher
- Manual For Students
Semester 2-2567
Summary
Course info
Cell Culture in Cosmetic Science
Teacher:
Nont Thitilertdecha
Skill Level
:
Beginner
